Contact an Account ManagerQuantitation of complex pharmaceutical raw materials
Summary
Type: HPLC
Application #: 101090
Detection: UV 254nm
Conditions: eluent: acetonitrile - 0.01M (NH4)2CO3 in water,
step1: 8% before injection,
step2: 15%, 1min,
step3: 30%, 1min,
step4: 40%, 3min,
step5: 50%, 2min,
step6: 60%, 4min,
flow rate 1.5ml/min,
pressure approx. 350bar,
injection volume 30μl.
Authors: Erni, F.
Source: J. Chromatogr. 125 (1976) 265–274
Details
Legend: Separation of ergot alkaloids,
1. lysergic acid, 2. isolysergic acid, 3. lysergic acid amide, 4. isolysergic
acid amide, 5. aci-ergotamine, 6. aci-ergotaminine, 7. ergotamine, 8.
ergotaminine;